o-Dianisidine dihydrochloride → Hexose

HRID 1255381

Reaktionsgleichung

Reaktionsgleichung von HRID 1255381
[OH:1][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4].COC1C=C(C2C=CC(N)=C(OC)C=2)C=CC=1N.Cl.Cl>P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+]>[CH2:11]([OH:12])[CH:9]1[O:10][CH:2]([OH:1])[CH:3]([OH:4])[CH:5]([OH:6])[CH:7]1[OH:8]

Komponenten

4

Edukt 2

HCID 62311o-Dianisidine dihydrochlorideHCID 64689Beta-D-Glucose

Produkt 1

HCID 206Hexose

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 24243Trisodium PhosphateLösungsmittel2×

Versuchsvorschrift

The assay was based on the method described by Sullivan and Ikawa (Biochimica et Biophysica Acta, 1973, 309:11-22), but modified to run in microtiter plates. An assay mixture contained 150 μl β-D-glucose (0.1 M in 0.1 M sodium phosphate buffer, pH 6.3), 120 μl 0.1 M sodium phosphate buffer, pH 6.3, 10 μl o-dianisidine-dihydrochloride (Sigma D-3252, 3.0 mg/ml in H2O), 10 μl peroxidase (POD) (Sigma P-8125, 0.1 ml in 0.1 M sodium phosphate buffer, pH 6.3) and 10 μl enzyme (HOX) solution. Blanks were made by adding buffer in place of enzyme solution.

Aufarbeitung

  1. additionby adding buffer in place of enzyme solution

Quellen und Nachweise

ORD-Eintrag
ord-ac4e11fff7634ccc90f49a0aade7629e
Quelldatensatz
uspto-grants-2013_06
Patentnummer
US08460723B2

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