Reaktionsgleichung
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].P([O-])([O-])([O-])=O>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]Komponenten
3
Versuchsvorschrift
To a glucose isomerase solution are added a 0.1 M D-glucose solution, a 0.05 M phosphate buffer solution and a 0.005 M MgSO4.7H2O solution. While maintaining at pH 7.0, the reaction is continued at 70° C. for one hour and the amount of fructose produced is measured. The unit of activity is expressed in terms of "unit" and the amount of enzyme which produces 1 mg of fructose under the above conditions is defined as 1 unit.
Aufarbeitung
- temperatureWhile maintaining at pH 7.0
Quellen und Nachweise
- ORD-Eintrag
- ord-63cd645593ce42c19eeaa11c501d5463
- Quelldatensatz
- uspto-grants-1981_03
- Patentnummer
- US04255521
Verknüpfte Notizen
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