(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → Ribosylthymidine diphosphate

HRID 1280309

Reaktionsgleichung

Reaktionsgleichung von HRID 1280309
[P:1]([O:13][CH2:14][C@H:15]1[O:19][C@@H:18]([N:20]2[CH:27]=[C:26]([CH3:28])[C:24](=[O:25])[NH:23][C:21]2=[O:22])[C@H:17]([OH:29])[C@@H:16]1[OH:30])([O:4][P:5]([O:8]P(O)(O)=O)([OH:7])=[O:6])(=[O:3])[OH:2].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[O-]P(OP([O-])([O-])=O)(=O)[O-].[O-]P(OP(OP([O-])([O-])=O)([O-])=O)(=O)[O-]>C(O)C(N)(CO)CO.S([O-])([O-])(=O)=O.[NH4+].[NH4+]>[P:1]([O:13][CH2:14][C@H:15]1[O:19][C@@H:18]([N:20]2[CH:27]=[C:26]([CH3:28])[C:24](=[O:25])[NH:23][C:21]2=[O:22])[C@H:17]([OH:29])[C@@H:16]1[OH:30])([O:4][P:5]([OH:8])([OH:7])=[O:6])(=[O:2])[OH:3]

Komponenten

7

Edukt 4

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 4513885-Methyluridine 5'-triphosphateHCID 644102Pyrophosphate2×HCID 3440921Triphosphate2×

Produkt 1

HCID 23379797Ribosylthymidine diphosphate

Reagenzien, Katalysatoren, Lösungsmittel 2

HCID 6503Tris(hydroxymethyl)aminomethaneLösungsmittel2×HCID 6097028Ammonium SulfateLösungsmittel

Versuchsvorschrift

5-Methyluridine 5′-triphosphate (0.0047 g, 0.009 mMol) was dissolved in 0.7 mL 1M Tris buffer (pH 8.5 containing 1M MgCl2) then 0.3 mL of a 0.25M glucose solution in 1M Tris buffer (pH 8.5 containing 1M MgCl2) added. A To timepoint was taken by reverse phase anion exchange HPLC. Hexokinase (EC 2.7.1.1, Boehringer Mannheim, from yeast overproducer), 75 units in 3.2M ammonium sulfate suspension, was added to the reaction which was incubated at 25° C. An HPLC was taken at 66 hours indicating complete conversion to diphosphate with no triphosphate remaining. Triphosphate retention: 17.5 minutes; diphosphate retention: 10.3 minutes.

Aufarbeitung

  1. customwas incubated at 25° C
  2. custom17.5 minutes
  3. custom10.3 minutes

Quellen und Nachweise

ORD-Eintrag
ord-f7b2247915384ecb88c46a7bdbef90cc
Quelldatensatz
uspto-grants-2002_09
Patentnummer
US06451288B1

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