Br[CH2:2][C@@H:3]1[NH:7][C:6](=[O:8])[CH2:5][CH2:4]1.[CH:9]1([O:14][C:15]2[CH:20]=[CH:19][C:18]([NH:21][C:22]([NH:24][C:25]3[CH:30]=[CH:29][C:28]([N:31]4[CH2:35][CH2:34][CH:33]([NH:36][CH3:37])[CH2:32]4)=[CH:27][CH:26]=3)=[O:23])=[CH:17][CH:16]=2)[CH2:13][CH2:12][CH2:11][CH2:10]1.C(=O)([O-])[O-].[K+].[K+]>CN(C=O)C>[CH:9]1([O:14][C:15]2[CH:16]=[CH:17][C:18]([NH:21][C:22]([NH:24][C:25]3[CH:30]=[CH:29][C:28]([N:31]4[CH2:35][CH2:34][CH:33]([N:36]([CH3:37])[CH2:2][C@H:3]5[CH2:4][CH2:5][C:6](=[O:8])[NH:7]5)[CH2:32]4)=[CH:27][CH:26]=3)=[O:23])=[CH:19][CH:20]=2)[CH2:13][CH2:12][CH2:11][CH2:10]1(R)-5-Bromomethylpyrrolidin-2-one (15 mg) was added to a suspension of 1-(4-cyclopentyloxyphenyl)-3-[4-(3-methyaminopyrrolidin-1-yl)phenyl]urea (30 mg) and potassium carbonate (20 mg) in DMF (3 ml). After 2 hours, the reaction mixture was filtered and concentrated, and the residue was purified by preparative HPLC. This resulted in the product with the molecular weight of 491.64 (C28H37N5O3); MS (ESI): 492 (M+H+) as hydrotrifluoroacetate.
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