S-adenosyl-L-methionine → 2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-1-benzopyran-3,5,7-triol

HRID 1359898

Reaktionsgleichung

Reaktionsgleichung von HRID 1359898
[CH2:1]1[C:10]2[C:5](=[CH:6][C:7]([OH:12])=[CH:8][C:9]=2[OH:11])[O:4][C@H:3]([C:13]2[CH:18]=[C:17](O)[C:16]([OH:20])=[C:15]([OH:21])[CH:14]=2)[C@@H:2]1[O:22]C(C1C=C(O)C(O)=C(O)C=1)=O.N[C@H](C(=O)O)CC[S+](C)C[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O>>[CH:18]1[C:13]([CH:3]2[O:4][C:5]3[CH:6]=[C:7]([OH:12])[CH:8]=[C:9]([OH:11])[C:10]=3[CH2:1][CH:2]2[OH:22])=[CH:14][C:15]([OH:21])=[C:16]([OH:20])[CH:17]=1

Komponenten

3

Edukt 2

HCID 34756S-adenosyl-L-methionineHCID 65064Epigallocatechin Gallate

Produkt 1

HCID 12032-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-1-benzopyran-3,5,7-triol

Versuchsvorschrift

The purified enzyme is suspended in a buffer having a pH of 4.5 to 8.5, preferably 6.5 to 8. To this suspension, EGCG or ECG is added along with S-adenosyl-L-methionine (SAM) and the reaction is allowed to proceed at 5 to 60° C., preferably at 20 to 40° C., to form a methylated catechin.

Aufarbeitung

  1. customto proceed at 5 to 60° C., preferably at 20 to 40° C.

Quellen und Nachweise

ORD-Eintrag
ord-af08bfc8067f41a89e53558cd6400aad
Quelldatensatz
uspto-grants-2011_02
Patentnummer
US07879586B2

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