Methamphetamine → Pseudoephedrine

HRID 1363911

Reaktionsgleichung

Reaktionsgleichung von HRID 1363911
[CH3:1][C@H:2]([NH:10][CH3:11])[CH2:3][C:4]1[CH:5]=[CH:6][CH:7]=[CH:8][CH:9]=1.C(=O)([O-])[O-:13].[Na+].[Na+].C(=O)(O)[O-]>>[CH3:1][C@H:2]([NH:10][CH3:11])[C@@H:3]([OH:13])[C:4]1[CH:5]=[CH:6][CH:7]=[CH:8][CH:9]=1

Komponenten

3

Edukt 2

HCID 10836Methamphetamine2×HCID 86624777Sodium carbonate bicarbonate

Produkt 1

HCID 7028Pseudoephedrine

Versuchsvorschrift

96-well microtitre plates were coated overnight at room temperature with monoclonal anti-methamphetamine antibody (East Coast Biologicals, USA) at 5 :g/ml, 100 :l/well, in 50 mM sodium carbonate/bicarbonate buffer, pH 9.6. The plate was washed 3 times with wash buffer and blocked in the same buffer for 30 minutes. 3-fold dilutions in assay buffer of the enzyme-drug conjugate (100 :l/well) were added. After 30 minutes incubation at room temperature, the plate was washed 3 times and the substrate solution added as described before. FIG. 4 shows the titration profile obtained with this conjugate. The conjugate retained both enzyme activity and the ability to bind to immobilised anti-methamphetamine antibody allowing its use in drug capture competitive enzyme immunoassay.

Aufarbeitung

  1. washThe plate was washed 3 times with wash buffer and
  2. customsame buffer for 30 minutes
  3. addition3-fold dilutions in assay buffer of the enzyme-drug conjugate (100 :l/well) were added
  4. washthe plate was washed 3 times
  5. additionthe substrate solution added
  6. customobtained with this conjugate

Quellen und Nachweise

ORD-Eintrag
ord-8f4c2cfafaad454e9fd8ecc5d471ad27
Quelldatensatz
uspto-grants-2011_02
Patentnummer
US07888040B2

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