Reaktionsgleichung
[NH2:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][CH2:4][CH2:5][NH:6][C:7](=N)[NH2:8].Cl.N[C@H](C(O)=[O:24])CCCNC(=N)N.C(OS(O)(=O)=O)CCCCCCCCCCC.N(CCO)(CCO)CCO>>[NH2:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][CH2:4][CH2:5][NH:6][C:7]([NH2:8])=[O:24]Komponenten
4
Reaktionsbedingungen
- Temperatur
- 37 °C
Versuchsvorschrift
[L-arginine deiminase activity is indicated in terms of micromoles of L-citrullin which are produced by reaction with L-arginine. The reaction with L-arginine is conducted by adding 10 g of the immobilized preparation to an aqueous 0.5 M L-arginine hydrochloride solution (pH 6.0) containing 0.01% of triethanolamine laurylsulfate, and shaking the mixture at 37° C. for one hour. The amount of L-citrullin produced is assayed colorimetrically by using diacetylmonooxim as a coloring agent.]
Aufarbeitung
- additionby adding
Quellen und Nachweise
- ORD-Eintrag
- ord-7b2d5f6cd6934ea9abd47800662b6fc3
- Quelldatensatz
- uspto-grants-1979_02
- Patentnummer
- US04138292
Verknüpfte Notizen
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