Tri-O-acetyl-D-glucal → HCID 14308117, Verbindung

Beteiligte Stoffe

Reaktionsschritte

7.0 g (30 mmol) of tri-O-acetylglucal in 70 ml of 0.25M phosphate buffer (pH=7) are stirred with 7 g of lipase from Pseudomonas spec. (lipase P, from Amano Pharmaceutical Co., Ltd., Nagoya, Japan) (free or immobilized on SiO2) at room temperature. After the reaction is complete (about 5-7 h) the reusable enzyme is separated off. The desired 4,6-di-O-acetylglucal is obtained either by extraction of the aqueous solution with CHCl3 or ethyl acetate or after freeze-drying and subsequent taking up in organic solvents such as ethyl acetate and filtering off the insoluble concomitants, The 4,6-di-O-acetylglucal which results in about 90% yield can be used without further purification for further reactions.

Herkunft & Nachweise