Dimethylformamide → (2S,4S)-1-(2-(1H-1,2,4-triazol-1-yl)acetyl)-4-((benzylamino)methyl)-N-(4-(4-fluorophenoxy)phenyl)pyrrolidine-2-carboxamide

Beteiligte Stoffe

Reaktionsschritte

Benzyl bromide (0.067 mL, 0.56 mmol) was added to a mixture of (2S,4S)-1-(2-(1H-1,2,4-triazol-1-yl)acetyl)-4-aminomethyl-N-(4-(4-fluorophenoxy)phenyl)pyrrolidine-2-carboxamide hydrochloride salt (0.247 g, 0.564 mmol), DMF (10 mL) and K2CO3 (0.312 g, 2.26 mmol), prepared as in Reference 12. The reaction mixture was stirred at ambient temperature for 30 minutes and then diluted with 1N hydrochloric acid (30 mL). The dilution was washed with ethyl acetate (30 mL). The aqueous layer was basified with 2N sodium hydroxide (40 mL) and extracted with methylene chloride (40 mL). The extract was washed with brine (20 mL), dried over anhydrous sodium sulfate, filtered and concentrated. Product was purified from the residue by reverse phase HPLC to give Compound 262, (2S,4S)-1-(2-(1H-1,2,4-triazol-1-yl)acetyl)-4-((benzylamino)methyl)-N-(4-(4-fluorophenoxy)phenyl)pyrrolidine-2-carboxamide (23 mg, 0.044 mmol, 8%), as an off-white solid. 1H-NMR (400 MHz, DMSO-d6): 10.03 (s, 1H), 8.44 (s, 1H), 7.96 (s, 1H), 7.58 (d, J=7.6 Hz, 2H), 7.37-7.30 (m, 3H), 7.25-7.17 (m, 3H), 7.03-6.94 (m, 5H), 5.30, 5.19 (m, 2H), 4.49-4.46 (m, 2H), 3.89-3.84 (m, 1H), 3.74 (s, 2H), 2.69-2.52 (m, 2H), 2.16-1.87 (m, 2H). MS (EI) for C29H29FN6O3. found 529.3 (MH+).

Herkunft & Nachweise