Potassium Phosphate, Tribasic → L-Ascorbic Acid

HRID 1655159

Reaktionsgleichung

Reaktionsgleichung von HRID 1655159
P([O-])([O-])([O-])=O.[K+].[K+].[K+].[CH2:9]([OH:20])[C@H:10]([OH:19])[C@H:11]1[O:16][C:14](=[O:15])[C@@H:13]([OH:17])[C@H:12]1[OH:18]>>[O:15]=[C:14]1[O:16][C@H:11]([C@H:10]([CH2:9][OH:20])[OH:19])[C:12]([OH:18])=[C:13]1[OH:17]

Komponenten

3

Edukt 2

HCID 62657Potassium Phosphate, TribasicHCID 439373L-Gulonic acid, gamma-lactone

Produkt 1

HCID 54670067L-Ascorbic Acid

Versuchsvorschrift

The reaction mixture containing 1.0 ml of cell free extract (protein content: 10.3 mg/ml) of Gluconobacter oxydans DSM 4025as prepared in the same manner as described in Example 1-(1) and (2), 1 ml of 0.5M potassium phosphate buffer (pH 7.0) and 0.5 ml of 17.8% L-gulono-gamma-lactone was incubated at 30° C. for 17.5 hours. As a result, 2.19 g/l of L-ascorbic acid was produced.

Aufarbeitung

  1. additionThe reaction mixture containing 1.0 ml of cell
  2. extractionfree extract (protein content: 10.3 mg/ml) of Gluconobacter oxydans DSM 4025as
  3. customprepared in the same manner

Quellen und Nachweise

ORD-Eintrag
ord-030d8550a1b44f86a259241a98a8ddd1
Quelldatensatz
uspto-grants-1993_10
Patentnummer
US05250428

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