Hydrochloric Acid → (S)-1-((4-(6-(4-chlorophenyl)-4-oxothieno[3,2-d]pyrimidin-3(4H)-yl)-2-methoxyphenoxy)methyl)-3,3-difluorocyclobutyl 2-aminopropanoate

A mixture of (S)-1-((4-(6-(4-chlorophenyl)-4-oxothieno[3,2-d]pyrimidin-3(4H)-yl)-2-methoxyphenoxy)methyl)-3,3-difluorocyclobutyl 2-(tert-butoxycarbonylamino)-propanoate from Part A (59 mg, 0.087 mmol) in 25% TFA/CH2Cl2 (4 mL) was stirred at RT for 30 min. After removal of the volatiles under vacuum, the crude product was purified by prep-HPLC (PHENOMENEX® Axia, Luna 5 micron 30×100 mm, flow rate=40 ml/min., gradient 0% A to 100% B in 10 min., A=90% H2O/10% MeOH/0.1% TFA, B=10% H2O/90% MeOH/0.1% TFA). The desired fractions were concentrated and dried under high vacao prior to addition of aq. saturated NaHCO3 (6 ml) and extraction with CH2Cl2 (2×10 ml). The combined CH2Cl2 layers were dried over Na2SO4 and concentrated prior to conversion of the free base (42 mg, 0.073 mmol) to the HCl salt by dissolution in CH2Cl2 (2 ml) and addition of 1.0 M HCl (0.079 mL, 0.079 mmol)/MeOH (2 ml) at −30° C. The HCl salt was then concentrated and was dried under high vacuum to yield (S)-1-((4-(6-(4-chlorophenyl)-4-oxothieno[3,2-d]pyrimidin-3(4H)-yl)-2-methoxyphenoxy)methyl)-3,3-difluorocyclobutyl 2-aminopropanoate (41.94 mg, 0.073 mmol, 83% yield) as white solid. 1H NMR (400 MHz, MeOD) δ ppm 8.27 (1H, s), 7.73 (2 H, d)3 7.63 (1 H, s), 7.43 (2 H, d), 7.02-7.15 (2 H, m), 6.94 (1 H, dd, J=8.56, 2.52 Hz), 4.41 (2 H, d, J=3.02 Hz), 3.91-4.02 (1 H, m), 3.78 (3 H, s), 2.87-3.18 (4 H, m), 1.44 (3 H, d, J=7.30 Hz). LC-MS: 2.33 min 576 (M+H). Luna 5 u C18 30×4.6 mm ID, flow rate=4 ml/min., gradient=0% A to 100% B in 2 min., A=90% H2O/10% MeOH/0.1% TFA, B=10% H2O/90% MeOH/0.1% TFA).

1. customAfter removal of the volatiles under vacuum
2. customthe crude product was purified by prep-HPLC (PHENOMENEX® Axia, Luna 5 micron 30×100 mm, flow rate=40 ml/min., gradient 0% A to 100% B in 10 min.
3. concentrationThe desired fractions were concentrated
4. customdried under high vacao
5. additionto addition of aq. saturated NaHCO3 (6 ml) and extraction with CH2Cl2 (2×10 ml)
6. dry with materialThe combined CH2Cl2 layers were dried over Na2SO4
7. concentrationconcentrated
8. concentrationThe HCl salt was then concentrated
9. customwas dried under high vacuum

ORD-Eintrag

ord-7dc39921c18d42fcb3e346ba6ca843cf

Quelldatensatz

uspto-grants-2011_08

DOI

10.6084/m9.figshare.5104873.v1

Patentnummer

US07989433B2