Sodium Chloride → Oleic Acid

HRID 1720128Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 1720128
[C:1]([O-:20])(=[O:19])[CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8]/[CH:9]=[CH:10]\[CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH3:18].[Na+].[Cl-].[Cl-].[Cl-].[Ca+2]>C1N(CCO)CCN(CCS(O)(=O)=O)C1>[C:1]([OH:20])(=[O:19])[CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8]/[CH:9]=[CH:10]\[CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH3:18]

Komponenten

5

Edukt 3

HCID 5234Sodium ChlorideHCID 5284359Calcium ChlorideHCID 5460221Oleic acid, ion(1-)

Produkt 1

HCID 445639Oleic Acid

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 238314-(2-Hydroxyethyl)-1-piperazineethanesulfonic acidLösungsmittel

Versuchsvorschrift

Purified human synovial fluid PLA2 (purified by the procedure of Fawzy, A. A. and Franson, R. C. Biophys. J. 49, 533a 1986!) was obtained from R. C. Franson (Medical College of Virginia, Richmond, Va.). PLA2 activity was measured by using 14C!-oleate labeled autoclaved E. coli as substrate as described by Franson, R. C., Patriarca, P., and Elsbach, P. J., Lipid Res. 15, 380-388 (1974). The assay was performed at 37° C for 30 min. in a final volume of 100 μL 50 mM Hepes buffer (pH 7.0) containing 150 mM NaCl, 5 mM CaCl2, and E. coli cells (corresponding to 10 mmol phospholipid). The test compound or control vehicle was preincubated with PLA2 for 5 min., followed by adding E. coli to initiate the reaction. The reaction was terminated by adding 2 mL tetrahydrofuran. The reaction product, 14C!-oleic acid, was isolated using a 1 mL Bond Elute--NH2 column and counted by liquid scintillation spectrometry.

Aufarbeitung

  1. customPurified human synovial fluid PLA2 (
  2. custompurified by the procedure of Fawzy
  3. custom) was obtained from R
  4. waitThe test compound or control vehicle was preincubated with PLA2 for 5 min.
  5. additionby adding E
  6. customthe reaction
  7. customThe reaction was terminated
  8. additionby adding 2 mL tetrahydrofuran

Quellen und Nachweise

ORD-Eintrag
ord-802b25f9e256463297ac7ffba2f2c10e
Quelldatensatz
uspto-grants-1996_04
Patentnummer
US05504237

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