L-Proline → cis-3-Hydroxy-L-proline

The enzyme reaction using purified L-proline-3-hydroxylase obtained in Example 5 was carried out. The reaction mixture was composed of 200 mM TES buffer (pH 7.0), 20 mM L-proline, 20 mM 2-ketoglutarate, 5 mM L-ascorbic acids 1 mM, ferrous sulfate, and 106 U of purified enzyme preparation, the total volume being 100 μl. The reaction was carried out at 35° C. for 30 minutes. As a result of the reaction, 18 mM (2.4 g/l) of cis-3-hydroxy-L-proline was produced in the reaction mixture.

1. customThe enzyme reaction
2. customusing purified L-proline-3-hydroxylase
3. customobtained in Example 5
4. customAs a result of the reaction

ORD-Eintrag

ord-3d0b07d8c4af47af92d4d4afbfaf4ad0

Quelldatensatz

uspto-grants-1999_10

DOI

10.6084/m9.figshare.5104873.v1

Patentnummer

US05962292