Sodium Carbonate → Biotin

HRID 1748841

Reaktionsgleichung

Reaktionsgleichung von HRID 1748841
[CH2:1]1[S:5][C@@H:4]([CH2:6][CH2:7][CH2:8][CH2:9][C:10](NCCCCCC(ON2C(=O)C(S([O-])(=O)=O)CC2=O)=O)=[O:11])[C@H:3]2[NH:32][C:33]([NH:35][C@@H:2]12)=[O:34].[Na+].C(=O)([O-])[O-:38].[Na+].[Na+]>>[OH:38][C:10]([CH2:9][CH2:8][CH2:7][CH2:6][C@H:4]1[C@@H:3]2[C@@H:2]([NH:35][C:33]([NH:32]2)=[O:34])[CH2:1][S:5]1)=[O:11]

Komponenten

3

Edukt 2

HCID 10340Sodium CarbonateHCID 71571497Sulphosuccinimidyl-6-(biotinamido)hexanoate

Produkt 1

HCID 171548Biotin

Versuchsvorschrift

The RA-NAH (10 mg) obtained in the above was dissolved in 0.5 ml of 0.1 M sodium carbonate aqueous solution, subsequently adding 3 mg of sulfo-NHS-LC-biotin (mfd, by Pierce Chemical Company) thereto followed by overnight incubation at 4° C. After overnight dialysis against distilled water thereafter, the dialyzed liquid was lyophilized to obtain the polysaccharide of the present invention in which biotin is bound to the reducing end of NAH (to be referred to as “NAH-biotin” hereinafter) (8 mg).

Aufarbeitung

  1. customThe RA-NAH (10 mg) obtained in the
  2. customat 4° C
  3. customAfter overnight dialysis
  4. distillationagainst distilled water

Quellen und Nachweise

ORD-Eintrag
ord-1b26caafa6a644a48874c7472e44e884
Quelldatensatz
uspto-grants-2015_10
Patentnummer
US09150902B2

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