Formate → Dihydroxyacetone Phosphate
Reaktionsgleichung
C([O-])=O.[Na+].[OH-].[Na+].C([O-])=O.C1N=C(N)C2N=CN([C@@H]3[O:23][C@H:22]([CH2:24][O:25][P:26]([O:29]P(OC[C@H]4O[C@@H](N5C=C(C(N)=O)CC=C5)[C@H](O)[C@@H]4O)(O)=O)([OH:28])=[O:27])[C@@H:21]([OH:51])[C@H]3O)C=2N=1>>[CH2:21]([OH:51])[C:22]([CH2:24][O:25][P:26]([OH:29])([OH:28])=[O:27])=[O:23]Komponenten
5
Versuchsvorschrift
For removing dX1P the acetaldehyde was evaporated and the solution was diluted with water to reach 30 ml. It was mixed with 3 ml 2.65 M sodium formate solution (8 mmol), and sodium hydroxide solution was added until a pH of 7.4 was reached. 23 U formate dehydrogenase (FDH), 6 mg NADH, 16 U RAMA and 20 U glycerolphosphate dehydrogenase (GDH) were added.