Octopamine → Dopamine

HRID 1839843

Reaktionsgleichung

Reaktionsgleichung von HRID 1839843
NCCC1C=CC(O)=CC=1.C1C(C(O)CN)=CC=C(O)C=1.[CH:22]1[C:27]([C@@H:28](O)[CH2:29][NH2:30])=[CH:26][C:25]([OH:32])=[C:24]([OH:33])[CH:23]=1>>[NH2:30][CH2:29][CH2:28][C:27]1[CH:22]=[CH:23][C:24]([OH:33])=[C:25]([OH:32])[CH:26]=1

Komponenten

4

Edukt 3

HCID 4581Octopamine4×HCID 5610Tyramine4×HCID 439260Norepinephrine3×

Produkt 1

HCID 681Dopamine

Versuchsvorschrift

Tyramine (TA), octopamine (OA) and norepinephrine (NE), naturally occurring monoamines that have a similar structure to DA, were tested together with DA for their ability to inhibit 3H-DA uptake. The concentration range of an unlabeled competitive inhibitor was selected according to the expected IC50 value. The IC50 values obtained were 0.39±0.11 μM for TA, 1.92±0.28 μM for OA, 4.10±1.02 μM for DA and 22.04±2.94 μM for NE. The apparent Ki values (calculation based on Cheng et al., (1973) Biochem Pharmacol 22:3099) were 0.35±0.10 μM for TA, 1.74±0.25 μM for OA, 3.72±0.92 μM for DA and 20.04±2.67 μM for The rank order of apparent Ki on OA/TAT for potential substrates was: TA<OA<DA<NE.

Aufarbeitung

  1. customThe IC50 values obtained

Quellen und Nachweise

ORD-Eintrag
ord-a659d6c4025b4dbf90c1ac258d98943a
Quelldatensatz
uspto-grants-2004_01
Patentnummer
US06677443B1

Verknüpfte Notizen

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