HCID 178467881, Verbindung → HCID 178461152, Verbindung

Beteiligte Stoffe

Reaktionsschritte

The biodegradable polymer of the present invention dissolved in N-methyl pyrollidone was loaded into a 1 ml syringe. Doxycycline hyclate was loaded into another 1 ml syringe to give a drug load of 8.5 percent doxycycline. The syringes were coupled together and mixed for 50 cycles. The syringe was then placed on a scale and tared. The composition containing doxycycline was then dropped into 5 ml phosphate buffer solution at a pH of 7.40 and a temperature of 37° C. The syringe was then placed back on the scale and the implant weight was recorded. This was repeated for each formulation four more times to give an n of five at each time point. The samples were then placed in an environmental shaker at 37° C. with a speed of 150 RPMs. At various time points, the phosphate buffer solution was decanted and the polymer implant was left in the vial. A fresh 5 ml of phosphate buffer solution was added to the precipitated polymer and placed back in the shaker. The phosphate buffer solution that was decanted was then analyzed by UV visibility for doxycycline content. Based upon the implant weight a theoretical amount of doxycycline was calculated. Drug release was then based upon this theoretical amount. Release data are depicted in FIG. 4.

Herkunft & Nachweise