Beteiligte Stoffe
| Rolle | Name | HCID | Einwaage |
|---|
| Edukt | Phenylmethylsulfonyl Fluoride | HCID 4784 | — |
| Edukt | 5,5'-Dithiobis(2-nitrobenzoic Acid) | HCID 6254 | — |
| Edukt | Bicinchoninic acid | HCID 71068 | — |
| Edukt | [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [3-hydroxy-2,2-dimethyl-4-oxo-4-[[3-oxo-3-(2-sulfanylethylamino)propyl]amino]butyl] hydrogen phosphate | HCID 439335 | — |
| Edukt | Dephospho-coa | HCID 444485 | — |
| Edukt | Riboflavin | HCID 493570 | — |
| Edukt | glutathionyl-coenzyme A | HCID 86755643 | — |
| Katalysator | Copper | HCID 23978 | — |
| Katalysator | Copper sulfate pentahydrate | HCID 24463 | — |
| Lösungsmittel | Tromethamine hydrochloride | HCID 93573 | — |
| Produkt | Coenzyme A | HCID 87642 | — |
Reaktionsschritte
Reduced nicotinamide adenine dinucleotide phosphate (NADPH), riboflavin, flavin adenine mononucleotide (FMN), flavin adenine dinucleotide (FAD), coenzyme A disulfide, glutathionyl-coenzyme A mixed disulfide, 3'-dephospho coenzyme A, 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB), lysostaphin, bicinchoninic acid solution, 4% copper sulphate pentahydrate solution, 2',5'-adenosine diphosphate (ADP)-Sepharose®; and phenylmethylsulphonyl fluoride (PMSF) were from Sigma (Mississauga, ON). 3'-dephospho CoA was oxidized to the disulfide by incubation at room temperature overnight in Tris-HCl (20 mM), pH 9.0, containing copper (5 μM). 4,4'-phosphopantetheine was formed by incubation of CoA with nucleotide pyrophosphatase. The thiol was oxidized as described above, and the disulfide was purified by high performance liquid chromatography (HPLC). All other chemicals were of reagent grade or better and were used without further purification.
Herkunft & Nachweise
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patentnummer | US06107068 |
|---|
| Quelldatensatz | uspto-grants-2000_08 |
|---|
| ORD ID | ord-20e171cb10bc4349a82014739271f945 |
|---|
| Quellennotiz | uspto-grants-2000_08 |
|---|
