NADP nicotinamide-adenine-dinucleotide phosphate → NADPH

HRID 1877742

Reaktionsgleichung

Reaktionsgleichung von HRID 1877742
[CH:1]1[CH:6]=[N+:5]([C@@H:7]2[O:11][C@H:10]([CH2:12][O:13][P:14]([O:17][P:18]([O:21][CH2:22][C@H:23]3[O:27][C@@H:26]([N:28]4[C:32]5[N:33]=[CH:34][N:35]=[C:36]([NH2:37])[C:31]=5[N:30]=[CH:29]4)[C@H:25]([O:38][P:39]([OH:42])([OH:41])=[O:40])[C@@H:24]3[OH:43])([OH:20])=[O:19])([OH:16])=[O:15])[C@@H:9]([OH:44])[C@H:8]2[OH:45])[CH:4]=[C:3]([C:46]([NH2:48])=[O:47])[CH:2]=1.P(OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)(O)(O)=O>C(O)C(N)(CO)CO>[CH:34]1[N:35]=[C:36]([NH2:37])[C:31]2[N:30]=[CH:29][N:28]([C@@H:26]3[O:27][C@H:23]([CH2:22][O:21][P:18]([O:17][P:14]([O:13][CH2:12][C@H:10]4[O:11][C@@H:7]([N:5]5[CH:4]=[C:3]([C:46]([NH2:48])=[O:47])[CH2:2][CH:1]=[CH:6]5)[C@H:8]([OH:45])[C@@H:9]4[OH:44])([OH:16])=[O:15])([OH:20])=[O:19])[C@@H:24]([OH:43])[C@H:25]3[O:38][P:39]([OH:42])([OH:41])=[O:40])[C:32]=2[N:33]=1

Komponenten

4

Edukt 2

HCID 5886NADP nicotinamide-adenine-dinucleotide phosphateHCID 439958Glucose-6-Phosphate

Produkt 1

HCID 5884NADPH

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 6503Tris(hydroxymethyl)aminomethaneLösungsmittel

Versuchsvorschrift

A mixture of substrate (5 mM), oxidized cofactor NADP+ (10 μM), glucose 6-phosphate (20 mM) in 50 mM Tris buffer pH 7.5 (0.8 ml) was admixed with G6PDH (10 u), after the enzyme (100-200 μg) had been added, and the reaction was carried out at 30° C. (140 rpm) for 48 hours.

Aufarbeitung

  1. additionafter the enzyme (100-200 μg) had been added
  2. customwas carried out at 30° C.
  3. customfor 48 hours

Quellen und Nachweise

ORD-Eintrag
ord-df688da077ca401b833773d89a7294a5
Quelldatensatz
uspto-grants-2012_11
Patentnummer
US08313923B2

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