HuagongsheHuagongshe Chemie-Druckbericht · Nur für Forschungszwecke
N-Succinimidyl 3-(2-pyridyldithio)propionate → Spdp dtt
Beteiligte Stoffe
| Rolle | Name | HCID | Einwaage |
|---|---|---|---|
| Edukt | N-Succinimidyl 3-(2-pyridyldithio)propionate | HCID 100682 | — |
| Edukt | Succinimidyl 4-(N-maleimidomethyl)cyclohexanecarboxylate | HCID 125175 | — |
| Edukt | D-1,4-Dithiothreitol | HCID 446094 | — |
| Produkt | Spdp dtt | HCID 131730073 | — |
Reaktionsschritte
SPDP-modified/DTT-reduced streptavidin is mixed with SMCC-modified BLA (equimolar ratio) and allowed to react for 1-2 hrs RT (or overnight at 4 degrees). The streptavidin-BLA conjugate product is purified by gel filtration over P-60 resin.
Herkunft & Nachweise
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patentnummer | US08318450B2 |
| Quelldatensatz | uspto-grants-2012_11 |
| ORD ID | ord-140178af5867432dbe2b7afad34eb7ab |
| Quellennotiz | uspto-grants-2012_11 |