o-Dianisidine dihydrochloride → Hexose

HRID 1919226

Reaktionsgleichung

Reaktionsgleichung von HRID 1919226
[OH:1][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4].COC1C=C(C2C=CC(N)=C(OC)C=2)C=CC=1N.Cl.Cl>P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+]>[CH2:11]([OH:12])[CH:9]1[O:10][CH:2]([OH:1])[CH:3]([OH:4])[CH:5]([OH:6])[CH:7]1[OH:8]

Komponenten

4

Edukt 2

HCID 62311o-Dianisidine dihydrochlorideHCID 64689Beta-D-Glucose

Produkt 1

HCID 206Hexose

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 24243Trisodium PhosphateLösungsmittel2×

Versuchsvorschrift

The assay was based on the method described by Sullivan and Ikawa (Biochimica et Biophysica Acta, 1973, 309:11-22), but modified to run in microtiter plates. An assay mixture contained 150 μl β-D-glucose (0.1 M in 0.1 M sodium phosphate buffer, pH 6.3), 120 μl 0.1 M sodium phosphate buffer, pH 6.3, 10 μl o-dianisidinedihydrochloride (Sigma D-3252, 3.0 mg/ml in H2O), 10 μl peroxidase (POD) (Sigma P-8125, 0.1 ml in 0.1 M sodium phosphate buffer, pH 6.3) and 10 μl enzyme (HOX) solution. Blanks were made by adding buffer in place of enzyme solution.

Aufarbeitung

  1. additionby adding buffer in place of enzyme solution

Quellen und Nachweise

ORD-Eintrag
ord-e9fbd9602cac49b4b66086316853ab93
Quelldatensatz
uspto-grants-2012_05
Patentnummer
USRE043341E1

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