DL-Arabinose → L-arabinono-1,4-lactone

Beteiligte Stoffe

Reaktionsschritte

Microbial synthesis begins with pentose oxidation using fermentor-controlled cultures (e.g., 1 L scale) of Pseudomonas fragi ATCC4973. (Buchert, J.; Viikari, L.; Linko, M.; Markkanen, P. Biotechnol. Lett. 1986, 8, 541. and Weimberg, R. J. Bio. Chem. 1961, 236, 629). D-xylose (100 g/L) is oxidized at 30° C. to D-xylonic acid and produces a 70% yield (77 g/L). L-arabinose is similarly oxidized and produces a 54% overall yield to a mixture of L-arabino-1,4-lactone (40 g/L) and L-arabinonic acid (15 g/L). The lactone is subsequently hydrolyzed to L-arabinonic acid. Escherichia coli constructs are then employed for the conversion of D-xylonic acid and L-arabinonic acid into the respective enantiomers of 1,2,4-butanetriol.

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