Diethylenetriaminepentaacetic acid → Dota

HRID 2108290

Reaktionsgleichung

Reaktionsgleichung von HRID 2108290
[C:1]([O-:4])(=[O:3])[CH3:2].[NH4+:5].[CH2:6]([N:17]([CH2:29][C:30]([OH:32])=[O:31])[CH2:18][CH2:19][N:20]([CH2:25][C:26](O)=O)[CH2:21][C:22]([OH:24])=[O:23])[CH2:7][N:8]([CH2:13][C:14](O)=O)[CH2:9][C:10]([OH:12])=[O:11]>>[CH2:26]1[N:5]([CH2:2][C:1]([OH:4])=[O:3])[CH2:14][CH2:13][N:8]([CH2:9][C:10]([OH:12])=[O:11])[CH2:7][CH2:6][N:17]([CH2:29][C:30]([OH:32])=[O:31])[CH2:18][CH2:19][N:20]([CH2:21][C:22]([OH:24])=[O:23])[CH2:25]1

Komponenten

3

Edukt 2

HCID 3053Diethylenetriaminepentaacetic acid2×HCID 517165Ammonium Acetate

Produkt 1

HCID 121841Dota

Versuchsvorschrift

Briefly, ammonium acetate, (10 μL for each mCi of 111In) is added to a reaction vial containing 111In-chloride solution. Subsequently, the DOTA-J591 solution (30 mL or 0.24 mg for each mCi of 111In) is added to the reaction vial and the mixture is gently mixed and incubated at 37° C. for 20-30 min. An aliquot of the mixture is tested to determine labeling efficiency using ITLC (SG and 5 mM DTPA, pH 5). If the binding is optimal (>70%), the reaction is stopped by the addition of 10-40 mL of 5 mM DTPA.

Aufarbeitung

  1. additionis gently mixed and incubated at 37° C. for 20-30 min

Quellen und Nachweise

ORD-Eintrag
ord-0b3f12f5cf1e43eb8de7b662dbe6e243
Quelldatensatz
uspto-grants-2009_04
Patentnummer
US07514078B2

Verknüpfte Notizen

Wird geladen…