Sodium Hydroxide → L-Lysine

HRID 2220013

Reaktionsgleichung

Reaktionsgleichung von HRID 2220013
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.OP([O-])(O)=O.[K+].[OH-].[Na+].S([O-])([O-])(=O)=O.[NH4+:26].[NH4+:27].Cl.S1C=CC=CC1.[OH:35][C:36]([CH2:38][CH2:39][CH2:40][CH2:41][C@H:42]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:37]>[Pt]>[NH2:26][C@H:38]([C:36]([OH:35])=[O:37])[CH2:39][CH2:40][CH2:41][CH2:42][NH2:27]

Komponenten

8

Edukt 6

HCID 14798Sodium HydroxideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 516951Monobasic potassium phosphateHCID 6097028Ammonium SulfateHCID 67590811Thiaminhydrochloride

Produkt 1

HCID 5962L-Lysine

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 23939PlatinumKatalysator

Reaktionsbedingungen

Temperatur
120 °C

Versuchsvorschrift

A medium containing 45 g/L of glucose, 15 g/L of molasses, 2 g/L (as nitrogen) of soybean protein hydrolysate, 2 g/L of KH2PO4, 5.6 g/L of NaOH, 10 g/L of ammonium sulfate, 0.8 g/L of MgSO4.7H2O, 20 mg/L of FeSO4.7H2O, 20 mg/L of MnSO4.4H2O, 0.8 mg/L of thiamin hydrochloride and 0.2 mg/L of biotin (pH 6.0) was introduced into 1-L volume small glass fermentation tank in a volume of 300 mL and sterilized by heating at 120° C. for 20 minutes. After the fermentation tank was cooled to 31.5° C, 5 platinum loops of Brevibacterium lactofermentum ATCC 31269 (refer to U.S. Pat. No. 4,066,501) preliminarily grown on an LB plate for 24 hours was inoculated to the medium and cultured at 31.5° C. and pH 7.0 for 30 hours with sufficient aeration and stirring.

Aufarbeitung

  1. temperatureAfter the fermentation tank was cooled to 31.5° C
  2. customcultured at 31.5° C.
  3. waitpH 7.0 for 30 hours with sufficient aeration

Quellen und Nachweise

ORD-Eintrag
ord-1b68ea4b5bc84dd5b31db6b4770dccc5
Quelldatensatz
uspto-grants-2008_08
Patentnummer
US07416740B2

Verknüpfte Notizen

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