O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.OP([O-])(O)=O.[K+].[OH-].[Na+].S([O-])([O-])(=O)=O.[NH4+:26].[NH4+:27].Cl.S1C=CC=CC1.[OH:35][C:36]([CH2:38][CH2:39][CH2:40][CH2:41][C@H:42]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:37]>[Pt]>[NH2:26][C@H:38]([C:36]([OH:35])=[O:37])[CH2:39][CH2:40][CH2:41][CH2:42][NH2:27]
A medium containing 45 g/L of glucose, 15 g/L of molasses, 2 g/L (as nitrogen) of soybean protein hydrolysate, 2 g/L of KH2PO4, 5.6 g/L of NaOH, 10 g/L of ammonium sulfate, 0.8 g/L of MgSO4.7H2O, 20 mg/L of FeSO4.7H2O, 20 mg/L of MnSO4.4H2O, 0.8 mg/L of thiamin hydrochloride and 0.2 mg/L of biotin (pH 6.0) was introduced into 1-L volume small glass fermentation tank in a volume of 300 mL and sterilized by heating at 120° C. for 20 minutes. After the fermentation tank was cooled to 31.5° C, 5 platinum loops of Brevibacterium lactofermentum ATCC 31269 (refer to U.S. Pat. No. 4,066,501) preliminarily grown on an LB plate for 24 hours was inoculated to the medium and cultured at 31.5° C. and pH 7.0 for 30 hours with sufficient aeration and stirring.
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