Phosphoric Acid → D-2-Aminohexano-6-lactam

HRID 2222061

Reaktionsgleichung

Reaktionsgleichung von HRID 2222061
[CH2:1]1[CH2:8][C@@H:7]([NH2:9])[C:5](=[O:6])[NH:4][CH2:3][CH2:2]1.CC1C(O)=C(C=O)C(COP(O)(O)=O)=CN=1.OP(O)(O)=O>>[CH2:1]1[CH2:8][C@H:7]([NH2:9])[C:5](=[O:6])[NH:4][CH2:3][CH2:2]1.[CH2:1]1[CH2:8][C@@H:7]([NH2:9])[C:5](=[O:6])[NH:4][CH2:3][CH2:2]1

Komponenten

5

Edukt 3

HCID 1004Phosphoric AcidHCID 1051Pyridoxal 5'-phosphateHCID 439823D-2-Aminohexano-6-lactam

Produkt 2

HCID 439823D-2-Aminohexano-6-lactamHCID 440599L-2-aminohexano-6-lactam

Versuchsvorschrift

Then D-α-amino-ε-caprolactam and pyridoxal-5-phosphate were added to the disintegrated cell suspensions of each monoculture to concentrations of 5 g/l and 0.01 mM respectively. After 1.5 h of incubation at 20° C. and 20 rpm, 2 ml samples were transferred into 1 ml of 1 M H3PO4. After removal of all particulates by centrifugation followed by filtration through a 0.22 μM filter, concentrations D- and L-α-amino-ε-caprolactam in the samples were determined by HPLC using the protocol as described in Example 1. Finally, monocultures that formed L-α-amino-ε-caprolactam from the D-α-amino-ε-caprolactam substrate were sent to the NCIMB for strain determination.

Aufarbeitung

  1. customAfter removal of all particulates by centrifugation
  2. filtrationfollowed by filtration through a 0.22 μM
  3. filtrationfilter
  4. concentrationconcentrations D- and L-α-amino-ε-caprolactam in the samples

Quellen und Nachweise

ORD-Eintrag
ord-588393c3f68845bbb4df505aac40a8bc
Quelldatensatz
uspto-grants-2008_05
Patentnummer
US07371555B2

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