(4S)-4-{[(benzyloxy)carbonyl]amino}-5-methoxy-5-oxopentanoic acid → Z-Glu-Ala-Ala-OH

Beteiligte Stoffe

Reaktionsschritte

0.2 ml of aqueous solution containing 2 mM Z-Glu-OMe, 50 mMH-Ala-Ala-OH, 25 mMNaOH and 5 mg/ml endoproteinase Glu-C are deep-frozen. Subsequently the reaction mixture is kept at -25° C. until the Z-Glu-OMe is consumed. Following thawing, the yield is determined analytically by RP-HPLC and amounts to 76% of theory.

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