Phosphate → Acridine Orange Ethidium Bromide

HRID 2264122

Reaktionsgleichung

Reaktionsgleichung von HRID 2264122
[CH3:1][N:2]([C:4]1[CH:5]=[CH:6][C:7]2[CH:8]=[C:9]3[C:14](=[N:15][C:16]=2[CH:17]=1)[CH:13]=[C:12]([N:18]([CH3:20])[CH3:19])[CH:11]=[CH:10]3)[CH3:3].[CH3:21][CH2:22][N+:23]1[C:36]([C:37]2[CH:38]=[CH:39][CH:40]=[CH:41][CH:42]=2)=[C:35]2[C:30]([CH:31]=[CH:32][C:33]([NH2:43])=[CH:34]2)=[C:29]2[C:24]=1[CH:25]=[C:26]([NH2:44])[CH:27]=[CH:28]2.[Br-:45].P([O-])([O-])([O-])=O>>[CH3:3][N:2]([C:4]1[CH:5]=[CH:6][C:7]2[CH:8]=[C:9]3[C:14](=[N:15][C:16]=2[CH:17]=1)[CH:13]=[C:12]([N:18]([CH3:20])[CH3:19])[CH:11]=[CH:10]3)[CH3:1].[CH3:21][CH2:22][N+:23]1[C:36]([C:37]2[CH:42]=[CH:41][CH:40]=[CH:39][CH:38]=2)=[C:35]2[C:30]([CH:31]=[CH:32][C:33]([NH2:43])=[CH:34]2)=[C:29]2[C:24]=1[CH:25]=[C:26]([NH2:44])[CH:27]=[CH:28]2.[Br-:45]

Komponenten

4

Edukt 3

HCID 1061PhosphateHCID 14710Ethidium Bromide2×HCID 62344Acridine Orange Base2×

Produkt 1

HCID 86616552Acridine Orange Ethidium Bromide

Versuchsvorschrift

Apoptosis was determined morphologically after staining with acridine orange and ethidium bromide and counting viable cells by fluorescence microscopy. Cell density was adjusted to 1×106 cells/ml in buffer. Acridine orange and ethidium bromide (1:1) (v/v) mixed in PBS (phosphate buffer solution) to a final concentration of 100 μg/ml. 2 μl dye was mixed with 25 μl of cell suspension and cells were immediately checked.

Aufarbeitung

  1. addition2 μl dye was mixed with 25 μl of cell suspension and cells

Quellen und Nachweise

ORD-Eintrag
ord-0d3710390add475597040889875ff99e
Quelldatensatz
uspto-grants-2010_12
Patentnummer
US07855073B2

Verknüpfte Notizen

Wird geladen…