Phosphate → Acridine Orange Ethidium Bromide
Reaktionsgleichung
[CH3:1][N:2]([C:4]1[CH:5]=[CH:6][C:7]2[CH:8]=[C:9]3[C:14](=[N:15][C:16]=2[CH:17]=1)[CH:13]=[C:12]([N:18]([CH3:20])[CH3:19])[CH:11]=[CH:10]3)[CH3:3].[CH3:21][CH2:22][N+:23]1[C:36]([C:37]2[CH:38]=[CH:39][CH:40]=[CH:41][CH:42]=2)=[C:35]2[C:30]([CH:31]=[CH:32][C:33]([NH2:43])=[CH:34]2)=[C:29]2[C:24]=1[CH:25]=[C:26]([NH2:44])[CH:27]=[CH:28]2.[Br-:45].P([O-])([O-])([O-])=O>>[CH3:3][N:2]([C:4]1[CH:5]=[CH:6][C:7]2[CH:8]=[C:9]3[C:14](=[N:15][C:16]=2[CH:17]=1)[CH:13]=[C:12]([N:18]([CH3:20])[CH3:19])[CH:11]=[CH:10]3)[CH3:1].[CH3:21][CH2:22][N+:23]1[C:36]([C:37]2[CH:42]=[CH:41][CH:40]=[CH:39][CH:38]=2)=[C:35]2[C:30]([CH:31]=[CH:32][C:33]([NH2:43])=[CH:34]2)=[C:29]2[C:24]=1[CH:25]=[C:26]([NH2:44])[CH:27]=[CH:28]2.[Br-:45]Komponenten
4
Versuchsvorschrift
Apoptosis was determined morphologically after staining with acridine orange and ethidium bromide and counting viable cells by fluorescence microscopy. Cell density was adjusted to 1×106 cells/ml in buffer. Acridine orange and ethidium bromide (1:1) (v/v) mixed in PBS (phosphate buffer solution) to a final concentration of 100 μg/ml. 2 μl dye was mixed with 25 μl of cell suspension and cells were immediately checked.