CFTRinh-172 → HCID 178418274, Verbindung
Reaktionsgleichung
[CH2:1]1[O:23][P:21]([OH:24])(=[O:22])[O:20][C@H:3]2[C@@H:4]([OH:19])[C@H:5]([N:7]3[C:17](Br)=[N:16][C:9]4[C:10]([N:12]=[C:13]([NH2:15])[NH:14][C:8]3=4)=[O:11])[O:6][C@H:2]12.C1C=C(N2C(=S)S/C(=C\C3C=CC(C(O)=O)=CC=3)/C2=O)C=C(C(F)(F)F)C=1>>[CH:17]1[N:7]([C@@H:5]2[O:6][C@@H:2]3[CH2:1][O:23][P:21]([OH:24])([O:20][C@H:3]3[C@H:4]2[OH:19])=[O:22])[C:8]2[NH:14][C:13]([NH2:15])=[N:12][C:10](=[O:11])[C:9]=2[N:16]=1Komponenten
3
Versuchsvorschrift
The result shown in FIG. 21 demonstrate that 8-Br-cGMP increases JV across hfRPE monolayers from 6.0 to 19.5 μl·cm−2·hr−1. This can be blocked by the addition of CFTR inhibitor CFTRinh-172, and a reduction in Jv from 19.5 to 11.5 μl·cm−2·hr−1 is observed. In four experiments, an increase in Jv from 7.4±1.1 to 15.8±1.6 μl·cm−2·hr−1 was observed, and addition of CFTR inhibitor reduced the increase in Jv to 8.9±1.3 μl·cm−2·hr−1.
Aufarbeitung
- temperatureincreases JV across hfRPE monolayers from 6.0 to 19.5 μl·cm−2·hr−1
- temperatureIn four experiments, an increase in Jv from 7.4±1.1 to 15.8±1.6 μl·cm−2·hr−1
- addition