HCID 605502, Verbindung → HCID 15612355, Verbindung

HRID 2371367Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 2371367
[CH3:1][O:2][C:3]([CH:5]1[C:12](=[O:13])[CH:11](C(OC)=O)[CH:10]2[CH:6]1[CH2:7][C:8](=[O:18])[CH2:9]2)=[O:4]>CO.P([O-])([O-])([O-])=O>[CH3:1][O:2][C:3]([CH:5]1[C:12](=[O:13])[CH2:11][CH:10]2[CH:6]1[CH2:7][C:8](=[O:18])[CH2:9]2)=[O:4]

Komponenten

4

Edukt 1

HCID 605502HCID 605502, Verbindung

Produkt 1

HCID 15612355HCID 15612355, Verbindung

Reagenzien, Katalysatoren, Lösungsmittel 2

HCID 887MethanolLösungsmittelHCID 1061PhosphateLösungsmittel

Versuchsvorschrift

One gram of 2,4-bismethoxycarbonylbicyclo[3.3.0]octane-3,7-dione is dissolved in 20 ml of methanol and combined with a solution of 2.5 g of α-chymotrypsin in 150 ml of 0.1-molar phosphate buffer pH 7. The solution is extracted with methyl isobutyl ketone after 22 hours of agitation at 28° C.; the extract is concentrated under vacuum, and the remaining residue is chromatographed over a silica gel column (gradient hexane/ethyl acetate), thus obtaining 610 mg of optically pure 2-methoxycarbonylbicyclo[3.3.0]octane-3,7-dione as an oily liquid.

Aufarbeitung

  1. extractionThe solution is extracted with methyl isobutyl ketone after 22 hours of agitation at 28° C.
  2. concentrationthe extract is concentrated under vacuum
  3. customthe remaining residue is chromatographed over a silica gel column (gradient hexane/ethyl acetate)

Quellen und Nachweise

ORD-Eintrag
ord-c9df848a40d0432db9a2709eb5c55621
Quelldatensatz
uspto-grants-1992_03
Patentnummer
US05093250

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