Pyridoxal 5'-phosphate → Ethylenediaminetetraacetic Acid

HRID 2380186

Reaktionsgleichung

Reaktionsgleichung von HRID 2380186
C(O)C(N)(CO)CO.[Na+].N[C@H](C([O-])=O)CCC([O-])=O.[Na+].[CH2:21]([N:32]([CH2:37][C:38]([O-:40])=[O:39])[CH2:33][C:34]([O-:36])=[O:35])[CH2:22][N:23]([CH2:28][C:29]([O-:31])=[O:30])[CH2:24][C:25]([O-:27])=[O:26].CC1N=CC(COP(O)(O)=O)=C(C=O)C=1O.CCC(COC(C(N(CC[NH+](C)C)C)=O)(C1C=CC=CC=1)C1C=CC=CC=1)CC.[Cl-].N[C@H](C([O-])=O)CC([O-])=O>O>[CH2:22]([N:23]([CH2:28][C:29]([OH:31])=[O:30])[CH2:24][C:25]([OH:27])=[O:26])[CH2:21][N:32]([CH2:37][C:38]([OH:40])=[O:39])[CH2:33][C:34]([OH:36])=[O:35]

Komponenten

8

Edukt 6

HCID 1051Pyridoxal 5'-phosphateHCID 6503Tris(hydroxymethyl)aminomethaneHCID 19153X 100HCID 164551EthylenediaminetetraacetateHCID 167560Disodium glutamateHCID 5460541l-aspartic acid, sodium salt

Produkt 1

HCID 6049Ethylenediaminetetraacetic Acid

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 962WaterLösungsmittel

Versuchsvorschrift

Dissolve 12.11 gm of Tris base, 50.72 gm of glutamic acid sodium salt, 1.86 gm of ethylenediaminetetraacetate (EDTA), 1.86 gm of polyvinyl pyrrolidone (PVP), 7.4 mg of pyridoxal phosphate (PP), and 50 mg of TRITON X-100 detergent (TX-100) in 1 liter of deionized water. Adjust the pH to 9.0. Add 1500 U of aspartate transaminase (AST) and mix well.

Aufarbeitung

  1. additionmix well

Quellen und Nachweise

ORD-Eintrag
ord-5d006b193f9945119432a295068647b6
Quelldatensatz
uspto-grants-1994_11
Patentnummer
US05366870

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