Phosphate Buffered Saline → Squalene

HRID 23859

Reaktionsgleichung

Reaktionsgleichung von HRID 23859
[C:1]([O-])(=O)[CH2:2][C:3]([CH2:8][C:9]([O-])=O)([C:5]([O-])=O)O.[CH2:14](O)[C:15](N)([CH2:18]O)[CH2:16]O.OP([O-])(O)=O.OP([O-])([O-])=O.[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]>>[CH3:14][C:15]([CH3:18])=[CH:16][CH2:1][CH2:2]/[C:3](/[CH3:5])=[CH:8]/[CH2:9][CH2:14]/[C:15](/[CH3:18])=[CH:16]/[CH2:1][CH2:9]/[CH:8]=[C:3](\[CH3:5])/[CH2:2][CH2:1]/[CH:16]=[C:15](\[CH3:18])/[CH2:14][CH2:9][CH:8]=[C:3]([CH3:5])[CH3:2]

Komponenten

3

Edukt 2

HCID 24978514Phosphate Buffered SalineHCID 92017416citrate Tris

Produkt 1

HCID 638072Squalene

Versuchsvorschrift

10 Baboons were divided into two groups (five baboons per group) and administered the formulations specified in Table 1. For Group 1, equal parts of the adjuvant MF59-0, and p24gag/sf2 (in a citrate/Tris buffer) were combined to yield a total of 0.7 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected intramuscularly (IM) in the thigh muscle. For Group 2, 1.5 ml of 2× phosphate buffered saline (PBS) was added to 46.8 mg of the PLG-entrapped p24gag/sf2 formulation produced in Example 1. The material was vortexed for about 30 seconds until all beads were in suspension. 1.5 ml of MF59-0 was added to the resuspended beads to yield a total of 3 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected IM in the thigh muscle.

Aufarbeitung

  1. customto yield a total of 0.7 ml
  2. additionThe composition was gently mixed
  3. customproduced in Example 1
  4. customto yield a total of 3 ml
  5. additionThe composition was gently mixed

Quellen und Nachweise

ORD-Eintrag
ord-324879adf1fa4d478af0321942615a58
Quelldatensatz
uspto-grants-2005_02
Patentnummer
US06855492B2

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