Phosphate Buffered Saline → Squalene
Reaktionsgleichung
[C:1]([O-])(=O)[CH2:2][C:3]([CH2:8][C:9]([O-])=O)([C:5]([O-])=O)O.[CH2:14](O)[C:15](N)([CH2:18]O)[CH2:16]O.OP([O-])(O)=O.OP([O-])([O-])=O.[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]>>[CH3:14][C:15]([CH3:18])=[CH:16][CH2:1][CH2:2]/[C:3](/[CH3:5])=[CH:8]/[CH2:9][CH2:14]/[C:15](/[CH3:18])=[CH:16]/[CH2:1][CH2:9]/[CH:8]=[C:3](\[CH3:5])/[CH2:2][CH2:1]/[CH:16]=[C:15](\[CH3:18])/[CH2:14][CH2:9][CH:8]=[C:3]([CH3:5])[CH3:2]Komponenten
3
Versuchsvorschrift
10 Baboons were divided into two groups (five baboons per group) and administered the formulations specified in Table 1. For Group 1, equal parts of the adjuvant MF59-0, and p24gag/sf2 (in a citrate/Tris buffer) were combined to yield a total of 0.7 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected intramuscularly (IM) in the thigh muscle. For Group 2, 1.5 ml of 2× phosphate buffered saline (PBS) was added to 46.8 mg of the PLG-entrapped p24gag/sf2 formulation produced in Example 1. The material was vortexed for about 30 seconds until all beads were in suspension. 1.5 ml of MF59-0 was added to the resuspended beads to yield a total of 3 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected IM in the thigh muscle.