HuagongsheHuagongshe Chemie-Druckbericht · Nur für Forschungszwecke
D-Alanine → DOTA biotin
Beteiligte Stoffe
| Rolle | Name | HCID | Einwaage |
|---|---|---|---|
| Edukt | D-Alanine | HCID 71080 | — |
| Edukt | N-Hydroxysuccinimide | HCID 80170 | — |
| Edukt | 1,3-Propanediamine, N'-(ethylcarbonimidoyl)-N,N-dimethyl-, monohydrochloride | HCID 2723939 | — |
| Edukt | Biotinyl-d-alanine | HCID 22863182 | — |
| Edukt | DOTA aniline | HCID 131719895 | — |
| Produkt | DOTA biotin | HCID 87364501 | — |
Reaktionsschritte
The D-alanine-linked conjugate was prepared by first coupling D-alanine (Sigma Chemical Co.) to biotin-NHS ester. The resultant biotinyl-D-alanine was then activated with 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (EDCI) and N-hydroxysuccinimide (NHS). This NHS ester was reacted in situ with DOTA-aniline to give the desired product which was purified by preparative HPLC.
Herkunft & Nachweise
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patentnummer | US06358490B2 |
| Quelldatensatz | uspto-grants-2002_03 |
| ORD ID | ord-de74687018da4a6989aa8c9929cb9295 |
| Quellennotiz | uspto-grants-2002_03 |