HuagongsheHuagongshe Chemie-Druckbericht · Nur für Forschungszwecke
Kanamycin → L-Phenylalanine
Beteiligte Stoffe
| Rolle | Name | HCID | Einwaage |
|---|---|---|---|
| Edukt | Kanamycin | HCID 6032 | — |
| Edukt | L-Leucine | HCID 6106 | — |
| Edukt | Trisodium citrate | HCID 6224 | — |
| Edukt | Potassium Phosphate, Dibasic | HCID 24450 | — |
| Edukt | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Edukt | Monobasic potassium phosphate | HCID 516951 | — |
| Edukt | HCID 178418600 | HCID 178418600 | — |
| Produkt | L-Phenylalanine | HCID 6140 | — |
Reaktionsschritte
The cell-suspension was transferred onto a minimum medium prepared by adding 5 μg/dl kanamycin, 10 mg/dl L-leucine, 10 mg/dl L-leucine, 10 mg/dl L-arginine, 100 mg/dl D, L-m-fluorophenylalanine and 2 g/dl agar to a basal minimum medium of pH 7.2 containing 0.6 g/dl KH2PO4, 1.4 g/dl K2HPO4, 0.2 g/dl (NH4)2SO4, 0.1 g/dl sodium citrate, 0.02 g/dl MgSO4.7H2O, and 0.5 g/dl glucose. After 3 days cultivation at 37° C., two colonies appeared on the agar-medium.
Herkunft & Nachweise
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patentnummer | US04591562 |
| Quelldatensatz | uspto-grants-1986_05 |
| ORD ID | ord-c3807252a2b846b6b79e083ef66b1ba9 |
| Quellennotiz | uspto-grants-1986_05 |