Biotin N-hydroxysuccinimide ester → Biotin

HRID 344635Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 344635
C1C(=O)N([O:8][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][C@@H:15]2[S:19][CH2:18][C@@H:17]3[NH:20][C:21]([NH:23][C@H:16]23)=[O:22])=[O:10])C(=O)C1>CN(C=O)C.C([O-])(O)=O.[Na+]>[OH:10][C:9]([CH2:11][CH2:12][CH2:13][CH2:14][C@H:15]1[C@@H:16]2[C@@H:17]([NH:20][C:21]([NH:23]2)=[O:22])[CH2:18][S:19]1)=[O:8]

Komponenten

4

Edukt 1

HCID 6710714Biotin N-hydroxysuccinimide ester4×

Produkt 1

HCID 171548Biotin

Reagenzien, Katalysatoren, Lösungsmittel 2

HCID 6228DimethylformamideLösungsmittelHCID 516892Sodium BicarbonateLösungsmittel

Versuchsvorschrift

Histone H1 was biotinylated to different degrees with increasing amounts of BHSE. 5 μg BHSE (dissolved in 10 μl DMF), 25 μg BHSE (in 10 μl DMF) and 250 μg BHSE in 10μl DMF were added to three solutions containing the same amount of histone H1 (1 mg in 300 μl of 50 mM NaHCO3) and incubated for 1 hour at 20° C. After dialysis against 5 mM sodium phosphate (pH 6.8) the three samples are stored at -20° C. The concentrations of the biotin-labelled histone H1 solutions are determined by comparison with unmodified histone H1 by SDS-polyacrylamide gel electrophoresis. The number of biotin residues in the three preparations was estimated by the tritium marker contained in BHSE and was found to be between 2.7 and 20 per histone H1 molecule.

Aufarbeitung

  1. customAfter dialysis against 5 mM sodium phosphate (pH 6.8) the three samples
  2. customare stored at -20° C

Quellen und Nachweise

ORD-Eintrag
ord-5bf0f635159d482cb89e9366520da456
Quelldatensatz
uspto-grants-1991_10
Patentnummer
US05053326

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