L-Aspartic Acid → N-[2-[(Acetylthio)methyl]-3,3,3-trifluoro-1-oxopropyl]-L-aspartic acid

Temperatur

0 °C

A stirred suspension of L-aspartic acid (2.66 g., 20 mmol.) in 35 mL of dry acetonitrile under argon was cooled to 0° C. and bis(trimethylsilyl)trifluoroacetamide (10.62 mL, 40 mmol) was added. The reaction mixture was allowed to stir and gradually warmed to room temperature overnight, and then 5 mL of dimethylformamide was added and the mixture was stirred for 2 hours. The resultant clear, light yellow solution was cooled to 5° C. and 2-trifluoromethyl-3-acetylthiopropionyl chloride (4.69 g., 20 mmol), dissolved in 6 mL of acetonitrile, was added dropwise. The reaction mixture was allowed to stir and warm to room temperature overnight, and then the solvent was evaporated. The yellow syrupy residue was partitioned between water (50 mL) and ethyl acetate (50 mL), and the organic layer was separated. The aqueous phase was further extracted with ethyl acetate (3×50 mL) and the combined ethyl acetate extract was washed with brine, dried (MgSO4) and evaporated to yield 12.2 g. of a light yellow residue. Flash chromatography on silica gel (125:1 silica gel to compound) eluting with ethyl acetate:acetic acid, 15:1 gave 5.25 g. of a major fraction which was a light yellow sticky solid and 0.63 g. fraction which was nearly homogeneous by TLC, for a total of 5.88 g. of the title compound as a mixture of diastereomers.

1. temperaturegradually warmed to room temperature overnight
2. stirringthe mixture was stirred for 2 hours
3. temperatureThe resultant clear, light yellow solution was cooled to 5° C.
4. additionwas added dropwise
5. stirringto stir
6. temperaturewarm to room temperature overnight
7. customthe solvent was evaporated
8. customThe yellow syrupy residue was partitioned between water (50 mL) and ethyl acetate (50 mL)
9. customthe organic layer was separated
10. extractionThe aqueous phase was further extracted with ethyl acetate (3×50 mL)
11. extractionthe combined ethyl acetate extract
12. washwas washed with brine
13. dry with materialdried (MgSO4)
14. customevaporated
15. customto yield 12.2 g
16. washFlash chromatography on silica gel (125:1 silica gel to compound) eluting with ethyl acetate
17. customacetic acid, 15:1 gave 5.25 g

ORD-Eintrag

ord-ca9ab82f383246dbada7d44138183f0d

Quelldatensatz

uspto-grants-1995_05

DOI

10.6084/m9.figshare.5104873.v1

Patentnummer

US05414013