Adenosine Monophosphate → HCID 178508926, Verbindung

HRID 373663

Reaktionsgleichung

[CH:1]1[N:5]([C@@H:6]2[O:9][C@H:8]([CH2:10][OH:11])[C@H:7]2[CH2:12][OH:13])[C:4]2[N:14]=[C:15]([NH2:19])[N:16]=[C:17](N)[C:3]=2[N:2]=1.[C@@H]1(N2C3N=CN=C(N)C=3N=C2)O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1[OH:22]>P([O-])([O-])([O-])=O>[CH:1]1[N:5]([C@@H:6]2[O:9][C@H:8]([CH2:10][OH:11])[C@H:7]2[CH2:12][OH:13])[C:4]2[NH:14][C:15]([NH2:19])=[N:16][C:17](=[O:22])[C:3]=2[N:2]=1

Komponenten

Reaktionsbedingungen

Temperatur: 37 °C

Versuchsvorschrift

After 2.0 g of 2-amino-OXT-A was dissolved in 300 ml of 1/10M phosphate buffer (pH 6.5), 8.0 g of 5'-adenylic acid deaminase (manufactured by Amano Pharmaceutical Co., Ltd., DEAMIZYME®) was added to the solution followed by stirring at 37° C. for 70 hours. After insoluble matters of the reaction solution were removed by filtration, the filtrate was passed through a column packed with 50 ml of activated charcoal powders (manufactured by Wako Pure Chemical Industry K.K., for chromatography). After washing with water, elution was performed with 50% aqueous methanol. By concentrating the desired fraction to dryness, crude OXT-G was obtained. The crude powders were dissolved in 50 ml of water and the OXT-G in the solution was adsorbed onto MCI® GEL CHP 20 (300 ml) followed by elution with water. The desired fraction was concentrated and evaporated to dryness under reduced pressure to give 1.5 g of OXT-G as colorless crystals (yield, 75%).

Aufarbeitung

additionwas added to the solution
customAfter insoluble matters of the reaction solution were removed by filtration
washAfter washing with water, elution
concentrationBy concentrating the desired fraction to dryness

Quellen und Nachweise

ORD-Eintrag: ord-152f04f6ff8c402b83461295500c4937
Quelldatensatz: uspto-grants-1992_11
DOI: 10.6084/m9.figshare.5104873.v1
Patentnummer: US05164500

Verknüpfte Notizen

Wird geladen…