Methanol → 4-(1-Nitrobenzyl)pyridine

HRID 383219

Reaktionsgleichung

Reaktionsgleichung von HRID 383219
[CH3:1]O.C1N=C(N)C2N=CN([C@@H]3O[C@H](COP(OP(OC[C@H]4O[C@@H]([N:33]5[CH:38]=[C:37](C(N)=O)[CH2:36][CH:35]=[CH:34]5)[C@H](O)[C@@H]4O)(O)=O)(O)=O)[C@@H](O)[C@H]3OP(O)(O)=O)C=2N=1.[N+:51]([C:54]1[CH:66]=[CH:65][C:57](CC2C=CN=CC=2)=[CH:56][CH:55]=1)([O-:53])=[O:52].[OH-].[Na+]>P([O-])([O-])([O-])=O.[K+].[K+].[K+].C(OC(=O)C)C.CC(C)=O>[N+:51]([C:54]1([CH:55]=[CH:56][CH:57]=[CH:65][CH2:66]1)[CH2:1][C:36]1[CH:35]=[CH:34][N:33]=[CH:38][CH:37]=1)([O-:53])=[O:52]

Komponenten

7

Edukt 4

HCID 887MethanolHCID 5884NADPHHCID 141294-(4-Nitrobenzyl)pyridineHCID 14798Sodium Hydroxide

Produkt 1

HCID 686711854-(1-Nitrobenzyl)pyridine

Reagenzien, Katalysatoren, Lösungsmittel 2

HCID 62657Potassium Phosphate, TribasicLösungsmittelHCID 21584569Ethylacetate acetoneLösungsmittel

Versuchsvorschrift

A typical reaction contained enzyme (1.0 ml, 1×10−6 M) in potassium phosphate buffer (0.1 M, pH 8.0), alkene (1.0×10−3 M), and methanol (1%) in a vial. The reaction was initiated with NADPH (50 μl, 1.0×10−3 M), and allowed to stir aerobically for 5 minutes. 300 μl of a stock solution of 4-(4-nitrobenzyl)pyridine (5% w/w in acetone) was added to the reaction, and the vial was sealed. The reaction was incubated at 80° C. for 20 minutes and chilled on ice. 600 μl of an ethylacetate/acetone (5:2) solution and 300 μl 5 M aqueous NaOH was added to the reaction solution. The solution was mixed thoroughly, and the absorbance of the organic layer was measured at 540 nm in a glass 96-well plate with microplate spectrophotometer.

Aufarbeitung

  1. customA typical reaction
  2. customthe vial was sealed
  3. waitThe reaction was incubated at 80° C. for 20 minutes
  4. temperaturechilled on ice
  5. additionThe solution was mixed thoroughly

Quellen und Nachweise

ORD-Eintrag
ord-6a2d41c281ab4b6aa97ff31a26cf2bba
Quelldatensatz
uspto-grants-2013_02
Patentnummer
US08367386B2

Verknüpfte Notizen

Wird geladen…