5-(1-Propynyl)-2'-deoxyuridine → 5-Propynyluracil

HRID 398798

Reaktionsgleichung

Reaktionsgleichung von HRID 398798
[C:1]([C:4]1[C:5](=[O:19])[NH:6][C:7](=[O:18])[N:8]([CH:17]=1)[C@@H]1O[C@H](CO)[C@@H](O)C1)#[C:2][CH3:3]>P([O-])([O-])([O-])=O>[C:1]([C:4]1[C:5](=[O:19])[NH:6][C:7](=[O:18])[NH:8][CH:17]=1)#[C:2][CH3:3]

Komponenten

3

Edukt 1

HCID 4802655-(1-Propynyl)-2'-deoxyuridine

Produkt 1

HCID 1318135-Propynyluracil

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 1061PhosphateLösungsmittel

Versuchsvorschrift

To a stirred solution of 2'-deoxy-5-propynyluridine (European Patent Specification No. 272065) (20g, 75 mmol) in aqueous phosphate buffer at pH 6.84 (1250 mL) was added purified E.coli thymidine phosphorylase (10,000 units) (T.A. Krenitsky et al, Biochemistry, 20, 3615, 1981; U.S. Patent Specification No. 4,381,344) and alkaline phosphatase (10,000 units) (Sigma type VII-S from bovine intestinal mucosa) and the whole mixture was incubated at 37° C. for 24 hours. The resulting white precipitate was filtered, washed with water (3 ×100 mL), ethanol (2 ×100 mL), ether (2 ×100 mL) and dried in vacuo over phosphorus pentoxide to give the title compound.

Aufarbeitung

  1. custompurified E.coli thymidine phosphorylase (10,000 units) (T.A. Krenitsky et al, Biochemistry, 20, 3615, 1981; U.S. Patent Specification No. 4,381,344) and alkaline phosphatase (10,000 units) (Sigma type VII-S from bovine intestinal mucosa)
  2. filtrationThe resulting white precipitate was filtered
  3. washwashed with water (3 ×100 mL), ethanol (2 ×100 mL), ether (2 ×100 mL)
  4. dry with materialdried in vacuo over phosphorus pentoxide

Quellen und Nachweise

ORD-Eintrag
ord-aa3ab387bbd140b7b484e3b00f1c21fc
Quelldatensatz
uspto-grants-1998_10
Patentnummer
US05817664

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