5-Fluorouracil → Floxuridine

HRID 429029

Reaktionsgleichung

Reaktionsgleichung von HRID 429029
[C@@H:1]1(N2C3N=CN=C(O)C=3N=C2)[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1.[F:19][C:20]1[C:21](=[O:27])[NH:22][C:23](=[O:26])[NH:24][CH:25]=1>C([O-])(=O)C.[NH4+]>[F:19][C:20]1[C:21](=[O:27])[NH:22][C:23](=[O:26])[N:24]([CH:25]=1)[C@@H:1]1[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1

Komponenten

4

Edukt 2

HCID 33855-FluorouracilHCID 178521064HCID 178521064, Verbindung

Produkt 1

HCID 5790FloxuridineAusbeute 30 %

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 517165Ammonium AcetateLösungsmittel

Versuchsvorschrift

An assay mixture containing 2 g (wet weight) of Bacillus stearothermophilus cells, 5 mM 2′-deoxyinosine and 5 mM 5-fluorouracil was made in 10 ml of 50 mM ammonium acetate buffer (pH 5.5). The conversions were run at 55° C. for 5 hour. The reaction mixture was centrifuged and then filtered using an Amicon Ultrafiltration device (YM-3 membrane) and the products were separated and analyzed by HPLC (C-18 column) in 5% methanol and 5 mM phosphate buffer at pH 4.6 using a flow rate of 0.5 ml/min. Blanks were run to check the authenticity of the reaction. No undesirable side reactions were detected. 5-fluoro-2′-deoxyuridine was obtained in 30% yield (62% conversion).

Aufarbeitung

  1. filtrationfiltered
  2. customthe products were separated
  3. customthe authenticity of the reaction

Quellen und Nachweise

ORD-Eintrag
ord-5b5cba3e459b4acc8ccf618ec487b114
Quelldatensatz
uspto-grants-2001_11
Patentnummer
US06316228B1

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