Reaktionsgleichung
[C:1]([OH:15])(=[O:14])[CH2:2][CH2:3][NH:4][C:5](=[O:13])[C@H:6]([C:8]([CH2:11][OH:12])([CH3:10])[CH3:9])[OH:7].S(=O)(=O)(O)O>>[C:1]([OH:15])(=[O:14])[CH2:2][CH2:3][NH:4][C:5](=[O:13])[C@@H:6]([C:8]([CH2:11][OH:12])([CH3:10])[CH3:9])[OH:7]Komponenten
Versuchsvorschrift
D-pantothenic acid was quantified by high performance liquid chromatography [column: Shimadzu SCR101H (7.9 mm dia.×30 cm); mobile phase: 0.008N sulfuric acid; flow rate: 0.8 ml/min; detector: differential refractometer] and/or microbial bioassay [indicator strain: Lactobacillus plantatum IFO 3070; medium: commercially available pantothenic acid assay medium (produced by DIFCO)]. Quantitative determination and optical purity determination of pantoic acid were conducted by high performance liquid chromatography [column: SUMICHIRAL OA-1200; mobile phase: n-hexane/1,2-dichloroethane/ethanol=90/8/2; flow rate: 1.0 ml/min; detector: UV] of the ethyl acetate extract after cell removal from the culture broth by centrifugation, addition of 6N hydrochloric acid to the supernatant and subsequent heating in a water bath at 80° C. for 15 minutes (by this operation, pantoic acid in an equilibrium is converted to pantolactone).
Quellen und Nachweise
- ORD-Eintrag
- ord-d5f92c09c85c4ea5ad2bd38b354d8e82
- Quelldatensatz
- uspto-grants-1996_05
- Patentnummer
- US05518906
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