4-Methylmorpholine → Z-Ile-Thr(tBu)-Thr(tBu)-Lys(Boc)-OPh

Beteiligte Stoffe

Reaktionsbedingungen

Reaktionsschritte

A solution of Z-Thr(tBu)-Thr(tBu)-Lys(Boc)-OPh (346.9 g, 450 mmol) in 1 l DMF was hydrogenated in the presence of Tos-OH.H2O (85.6 g, 450 mmol) and 10% Pd-C. After the hydrogenation was completed the catalyst was filtered off. Z-Ile-OH (132.66, 500 mmol) was dissolved in 700 ml DMF and cooled to -35° C. While stirring at -35° C., N-methylmorpholine (55 ml, 500 mmol) was added, followed by the addition of isobutyl chloroformate (65 ml, 500 mmol). The reaction mixture was activated for 5 min. at -15° C. and quickly cooled to -20° C. The precooled DMF solution (-25° C.) from the hydrogenation step and N-methylmorpholine (50 ml, 450 mmol) were added simultaneously. The reaction mixture was stirred for 15 min. at -15° C. and 1 h at 25° C. and evaporated in vacuo. The residue was dissolved in 3 l ethyl acetate and washed with 10% Na2CO3 sol. (2×1.5 l), satd. NaCl-sol. (1×1 l), 5% KHSO4 /10% K2SO4 sol. (2×1.5 l) and satd. NaCl-sol., dried over Na2SO4, filtered and evaporated in vacuo. The residue was crystallized from ethyl acetate (2 l)/ether (2 l).

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