Nadide (JAN/USAN/INN) → NADPH

HRID 483769

Reaktionsgleichung

Reaktionsgleichung von HRID 483769
[CH:1]1[N:2]=[C:3]([NH2:44])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([O:20][P:21]([O:24][CH2:25][C@H:26]4[O:30][C@@H:29]([N:31]5[CH:36]=[C:35]([C:37]([NH2:39])=[O:38])[CH2:34][CH:33]=[CH:32]5)[C@H:28]([OH:40])[C@@H:27]4[OH:41])([OH:23])=[O:22])([OH:19])=[O:18])[C@@H:12]([OH:42])[C@H:11]3[OH:43])[C:5]=2[N:6]=1.[CH:45]1[CH:50]=[N+:49]([C@@H:51]2[O:55][C@H:54]([CH2:56][O:57][P:58]([O:61][P:62]([O:65][CH2:66][C@H:67]3[O:71][C@@H:70]([N:72]4[C:76]5[N:77]=[CH:78][N:79]=[C:80]([NH2:81])[C:75]=5[N:74]=[CH:73]4)[C@H:69]([OH:82])[C@@H:68]3[OH:83])([OH:64])=[O:63])([OH:60])=[O:59])[C@@H:53]([OH:84])[C@H:52]2[OH:85])[CH:48]=[C:47]([C:86]([NH2:88])=[O:87])[CH:46]=1>>[CH:1]1[N:2]=[C:3]([NH2:44])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([O:20][P:21]([O:24][CH2:25][C@H:26]4[O:30][C@@H:29]([N:31]5[CH:36]=[C:35]([C:37]([NH2:39])=[O:38])[CH2:34][CH:33]=[CH:32]5)[C@H:28]([OH:40])[C@@H:27]4[OH:41])([OH:23])=[O:22])([OH:19])=[O:18])[C@@H:12]([OH:42])[C@H:11]3[O:43][P:58]([OH:60])([OH:59])=[O:57])[C:5]=2[N:6]=1.[CH:45]1[CH:50]=[N+:49]([C@@H:51]2[O:55][C@H:54]([CH2:56][O:57][P:58]([O:61][P:62]([O:65][CH2:66][C@H:67]3[O:71][C@@H:70]([N:72]4[C:76]5[N:77]=[CH:78][N:79]=[C:80]([NH2:81])[C:75]=5[N:74]=[CH:73]4)[C@H:69]([O:82][P:17]([OH:19])([OH:18])=[O:16])[C@@H:68]3[OH:83])([OH:64])=[O:63])([OH:60])=[O:59])[C@@H:53]([OH:84])[C@H:52]2[OH:85])[CH:48]=[C:47]([C:86]([NH2:88])=[O:87])[CH:46]=1

Komponenten

4

Edukt 2

HCID 5893Nadide (JAN/USAN/INN)HCID 439153Nadh

Produkt 2

HCID 5884NADPHHCID 5886NADP nicotinamide-adenine-dinucleotide phosphate

Reaktionsbedingungen

Temperatur
100 °C

Versuchsvorschrift

To 0.9 ml of each of the above substrate solutions containing NADH or NAD+ was added 0.1 ml of 23 U/ml of the present enzyme, and the reaction was carried out at 30° C. for 20 minutes. Then, the reaction was terminated by heat treatment at 100° C. for 2 minutes, and the denatured protein was removed, after which the amount of NADPH or NADP+ produced by the reaction of the corresponding substrate was measured as follows. As a control solution, there was used a mixture of each substrate solution and the present enzyme in which the reaction had been terminated immediately after mixing them.

Aufarbeitung

  1. customThen, the reaction was terminated
  2. customthe denatured protein was removed

Quellen und Nachweise

ORD-Eintrag
ord-780ec6230df3424eb00f2854834671cc
Quelldatensatz
uspto-grants-1993_07
Patentnummer
US05227299

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