O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.S([O-])([O-])(=O)=O.[NH4+:18].[NH4+:19].[K].S([O-])([O-])(=O)=O.[Mg+2].CC1[N+](C[C:34]2[CH:35]=[N:36][C:37](C)=[N:38][C:39]=2N)=CSC=1CCO.Cl.[Cl-].[OH:47][C:48]([CH2:50]CCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:49]>S([O-])([O-])(=O)=O.[Mn+2]>[NH2:18][C@H:50]([C:48]([OH:47])=[O:49])[CH2:35][CH2:34][CH2:39][NH:38][C:37](=[NH:36])[NH2:19]
An aqueous solution medium having a composition of 10% of glucose, 4% of ammonium sulfate, 0.1% of potassium primary phosphate, 0.04% of magnesium sulfate, 0.001% of ferrous sulfate, 0.001% of manganese sulfate, 100 μg/l of thiamine hydrochloride, 100 μg/l of biotin, 5 ml/dl of Aji-Eki® and 0.2% of yeast extract, at pH 7.0, was charged in an amount of 300 ml into separate small sized glass jar fermenters. After sterilizing in a conventional manner, the various L-arginine-producing bacterial strains shown in Table 12, which had been previously grown in bouillon slants at 30° C. for 24 hours, were inoculated thereon. Then, incubation was carried out at 31.5° C. for 48 hours at 1200 rpm at an aeration rate of 1/2 volume per minute, while keeping the pH at 7.0 by the addition of ammonia gas. After completion of the fermentation, the yield of L-arginine produced and accumulated in the solution based on glucose was determined and is shown in Table 12.
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