Pyruvate → 3-Deoxy-L-glycero-L-galacto-2-nonulosonic acid

Beteiligte Stoffe

Reaktionsbedingungen

Reaktionsschritte

To a 10 mL potassium phosphate buffer (0.1 M, pH 7.5) were added 10 mM DTT, 0.5 M pyruvate, 0.1 M D-mannose and 1.5 mg NeuAc aldolase. The reaction mixture was shaken at 37° C. for three days. The reaction mixture was chromatographed with a Dowex-1 (HCO3−) resin, eluted with zero to 1 M ammonium bicarbonate gradient. The fractions containing KDN were pooled and lyophilized three times repeatedly to remove the volatile salt to give a 78 percent yield of KDN. The physical data of the product were are consistent with the reported.

Herkunft & Nachweise