o-Dianisidine dihydrochloride → Hexose

HRID 586103

Reaktionsgleichung

Reaktionsgleichung von HRID 586103
[OH:1][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4].COC1C=C(C2C=CC(N)=C(OC)C=2)C=CC=1N.Cl.Cl>P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+]>[CH2:11]([OH:12])[CH:9]1[O:10][CH:2]([OH:1])[CH:3]([OH:4])[CH:5]([OH:6])[CH:7]1[OH:8]

Komponenten

4

Edukt 2

HCID 62311o-Dianisidine dihydrochlorideHCID 64689Beta-D-Glucose

Produkt 1

HCID 206Hexose

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 24243Trisodium PhosphateLösungsmittel2×

Versuchsvorschrift

The assay was based on the method described by Sullivan and Ikawa (Biochimica et Biophysica Acta, 1973, 309:11-22), but modified to run in microtiter plates. An assay mixture contained 150 μl β-D-glucose (0.1 M in 0.1 M sodium phosphate buffer, pH 6.3), 120 μl 0.1 M sodium phosphate buffer, pH 6.3, 10 μl o-dianisidine-dihydrochloride (Sigma D-3252, 3.0 mg/ml in H2O), 10 μl peroxidase (POD) (Sigma P-8125, 0.1 ml in 0.1 M sodium phosphate buffer, pH 6.3) and 10 μl enzyme (HOX) solution. Blanks were made by adding buffer in place of enzyme solution.

Aufarbeitung

  1. additionby adding buffer in place of enzyme solution

Quellen und Nachweise

ORD-Eintrag
ord-654a2f39290e4a00ac02280cb85320bb
Quelldatensatz
uspto-grants-2005_08
Patentnummer
US06936289B2

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