Vitamin D3 → 7-Dehydrocholesterol

HRID 68176

Reaktionsgleichung

Reaktionsgleichung von HRID 68176
[CH3:1][C@@H:2]([C@@H:9]1[C@@:13]2([CH3:28])[CH2:14][CH2:15][CH2:16]/[C:17](=[CH:18]\[CH:19]=[C:20]3\[CH2:21][C@@H:22]([OH:27])[CH2:23][CH2:24][C:25]\3=[CH2:26])/[C@@H:12]2[CH2:11][CH2:10]1)[CH2:3][CH2:4][CH2:5][CH:6]([CH3:8])[CH3:7].C[C@@H]([C@@H]1[C@@]2(C)CCC/C(=C\C=C3\C[C@@H](O)CCC\3=C)/[C@@H]2CC1)CCCC(O)(C)C>C(O)C>[CH3:1][C@@H:2]([C@@H:9]1[C@@:13]2([CH3:28])[CH2:14][CH2:15][C@@H:16]3[C@@:25]4([CH3:26])[CH2:24][CH2:23][C@H:22]([OH:27])[CH2:21][C:20]4=[CH:19][CH:18]=[C:17]3[C@@H:12]2[CH2:11][CH2:10]1)[CH2:3][CH2:4][CH2:5][CH:6]([CH3:7])[CH3:8]

Komponenten

4

Edukt 2

HCID 5280795Vitamin D35×HCID 528373125-Hydroxyvitamin D3

Produkt 1

HCID 4394237-Dehydrocholesterol

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 702EthanolLösungsmittel3×

Versuchsvorschrift

For the treatment with vitamin D metabolites, stock solutions of 2 mM 7-Dehydrocholesterol (7-DHC, Sigma St. Louis, Mo., USA), 2 μM cholecalciferol (D3, Sigma St. Louis, Mo., USA), and 2 μM 25-Hydroxyvitamin D (25-D3, Sigma St. Louis, Mo., USA) were prepared in absolute ethanol and filtered with a 0.22 mm pore size filter before use. Fourteen different groups were prepared; eight were non irradiated: 7-DHC at different doses (20 nmols, 2 nmols, 0.2 nmols, 2×10−2 nmols, and 2×10−3 nmols), 2×10−2 nmols D3, 2×10−2 nmols 25-D3 and ethanol. And six were UV-irradiated: 7-DHC at the same different doses (20 nmols, 2 nmols, 0.2 nmols, 2×10−2 nmols, and 2×10−3 nmols) and ethanol.

Aufarbeitung

  1. filtrationfiltered with a 0.22 mm pore size
  2. filtrationfilter before use
  3. customFourteen different groups were prepared
  4. customeight were non irradiated

Quellen und Nachweise

ORD-Eintrag
ord-a2dadd22025a409987662a0f64ebd88e
Quelldatensatz
uspto-grants-2014_09
Patentnummer
US08821568B2

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