2-Mercaptoethanol → 4-Methylumbelliferone

HRID 736589

Reaktionsgleichung

Reaktionsgleichung von HRID 736589
SCCO.C(N(CC([O-])=O)CC(O)=O)CN(CC([O-])=O)CC(O)=O.[Na+].[Na+].CCCCCCCCCCCCOS([O-])(=O)=O.[Na+].CCC(COC(C(N(CC[NH+](C)C)C)=O)(C1C=CC=CC=1)C1C=CC=CC=1)CC.[Cl-].[CH3:75][C:76]1[C:82]2[CH:83]=[CH:84][C:85]([O:87][C@@H]3O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]3O)=[CH:86][C:81]=2[O:80][C:78](=[O:79])[CH:77]=1>C([O-])([O-])=O.[Na+].[Na+]>[CH3:75][C:76]1[C:82]2[CH:83]=[CH:84][C:85]([OH:87])=[CH:86][C:81]=2[O:80][C:78](=[O:79])[CH:77]=1

Komponenten

7

Edukt 5

HCID 15672-MercaptoethanolHCID 8759EDTA disodium saltHCID 19153X 100HCID 915534-Methylumbelliferyl beta-D-glucuronideHCID 3423265Sodium Lauryl Sulfate

Produkt 1

HCID 52805674-Methylumbelliferone

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 10340Sodium CarbonateLösungsmittel

Versuchsvorschrift

To evaluate the activity of various promoters, GUS fluorescence was assayed by the method of Jefferson (1987). Maturing seeds at 17 DAF were homogenized in GUS extraction buffer (50 mM NaPO4 [pH 7.0], 10 mM 2-mercaptoethanol, 10 mM Na2-EDTA, 0.1% SDS, 0.1% Triton X-100). After centrifugation, 10 μl of the supernatant was mixed with 90 μl of assay buffer containing 1 mM 4-methylumbelliferyl-β-D-glucuronide (MUG). After incubating for one hour at 37° C., 900 μl of 0.2 M Na2CO3 was added to the mixture to terminate the reaction. Values obtained using a fluorometer were compared with those obtained from serial dilutions of 4-methylumbelliferone (4MU). The protein amount was determined using a BIG-RAD Protein Assay kit, with serum albumin as the standard. Three seeds were assayed for each transgenic plant.

Aufarbeitung

  1. additionAfter centrifugation, 10 μl of the supernatant was mixed with 90 μl of assay
  2. customto terminate
  3. customthe reaction
  4. customValues obtained

Quellen und Nachweise

ORD-Eintrag
ord-9635d93be8f2419c88ab4c58f6dc1584
Quelldatensatz
uspto-grants-2009_09
Patentnummer
US07595384B2

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