Phosphate Buffered Saline → Squalene

HRID 746355

Reaktionsgleichung

Reaktionsgleichung von HRID 746355
[C:1]([O-])(=O)[CH2:2][C:3]([CH2:8][C:9]([O-])=O)([C:5]([O-])=O)O.[CH2:14](O)[C:15](N)([CH2:18]O)[CH2:16]O.OP([O-])(O)=O.OP([O-])([O-])=O.[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]>>[CH3:14][C:15]([CH3:18])=[CH:16][CH2:1][CH2:2]/[C:3](/[CH3:5])=[CH:8]/[CH2:9][CH2:14]/[C:15](/[CH3:18])=[CH:16]/[CH2:1][CH2:9]/[CH:8]=[C:3](\[CH3:5])/[CH2:2][CH2:1]/[CH:16]=[C:15](\[CH3:18])/[CH2:14][CH2:9][CH:8]=[C:3]([CH3:5])[CH3:2]

Komponenten

3

Edukt 2

HCID 24978514Phosphate Buffered SalineHCID 92017416citrate Tris

Produkt 1

HCID 638072Squalene

Versuchsvorschrift

10 Baboons were divided into two groups (five baboons per group) and administered the formulations specified in Table 1. For Group 1, equal parts of the adjuvant MF59-0, and p24gag/sf2 (in a citrate/Tris buffer) were combined to yield a total of 0.7 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected intramuscularly (IM) in the thigh muscle. For Group 2, 1.5 ml of 2× phosphate buffered saline (PBS) was added to 46.8 mg of the PLG-entrapped p24gag/sf2 formulation produced in Example 1. The material was vortexed for about 30 seconds until all beads were in suspension. 1.5 ml of MF59-0 was added to the resuspended beads to yield a total of 3 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected IM in the thigh muscle.

Aufarbeitung

  1. customto yield a total of 0.7 ml
  2. additionThe composition was gently mixed
  3. customproduced in Example 1
  4. customto yield a total of 3 ml
  5. additionThe composition was gently mixed

Quellen und Nachweise

ORD-Eintrag
ord-b2f7a63f9a06431dac68f45da5d4ecf1
Quelldatensatz
uspto-grants-2008_07
Patentnummer
US07393630B2

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