Reaktionsgleichung
[NH2:1][C@@H:2]([C:5]([OH:7])=[O:6])[CH2:3][SH:4].[C:8]([C:10]1[S:11][C:12]2[CH:18]=[C:17]([OH:19])[CH:16]=[CH:15][C:13]=2[N:14]=1)#N>>[CH2:3]1[S:4]/[C:8](=[C:10]2/[N:14]=[C:13]3[C:12]([S:11]/2)=[CH:18][C:17](=[O:19])[CH:16]=[CH:15]3)/[NH:1][C@H:2]1[C:5]([OH:7])=[O:6]Komponenten
3
Versuchsvorschrift
First, in Step 1, a reagent for quantitating D-cysteine is added to a sample. If D-cysteine is present in the sample, it reacts with 2-cyano-6-hydroxybenzothiazole to produce D-luciferin. Then, in Step 2, a bioluminescent reagent is added to the sample to measure the generated luminescent level with a luminometer. Finally, the D-cysteine level in the sample is calculated from the luminescent level based on the calibration curve prepared beforehand.
Aufarbeitung
- additionis added to a sample
Quellen und Nachweise
- ORD-Eintrag
- ord-30851465ed094977a366bba1f815b529
- Quelldatensatz
- uspto-grants-2002_04
- Patentnummer
- US06376208B1
Verknüpfte Notizen
Wird geladen…