Ammonia → 2,6-Diamino-9-(2-hydroxyethoxymethyl)purine

HRID 848084Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 848084
Cl[C:2]1[N:10]=[C:9]2[C:5]([N:6]=[CH:7][N:8]2[CH2:11][O:12][CH2:13][CH2:14][OH:15])=[C:4]([NH2:16])[N:3]=1.[NH3:17]>>[NH2:17][C:2]1[N:10]=[C:9]2[C:5]([N:6]=[CH:7][N:8]2[CH2:11][O:12][CH2:13][CH2:14][OH:15])=[C:4]([NH2:16])[N:3]=1

Komponenten

3

Edukt 2

HCID 222AmmoniaHCID 128995862-Chloro-9-(2-hydroxyethoxymethyl)adenine

Produkt 1

HCID 945882,6-Diamino-9-(2-hydroxyethoxymethyl)purineAusbeute 12 %

Reaktionsbedingungen

Temperatur
120 °C

Versuchsvorschrift

A mixture of 2-chloro-9-(2-hydroxyethoxymethyl)adenine (4.4 g) and liquid ammonia (40 ml) was heated at 120° C. in a bomb for 19 hours. The reaction mixture was removed from the bomb and excess ammonia removed in vacuo. The residue was partitioned between ether and water and the aqueous layer washed twice more with ether and once with chloroform. The aqueous solution was then treated with an excess of a strongly basic ion exchange resin to convert the ammonium chloride to ammonia and evaporated to dryness in vacuo. The residue was dissolved in a minimal amount of ethanol, Florisil (Registered Trade Mark) (10g) added, and the mixture evaporated in dryness. The residue was transferred to a prepared column of Florisil in chloroform and eluted with 5% methanol/95% chloroform (v/v). Fractions (30 ml) were collected and examined by TLC (silica gel plates developed in 10% methanol/90% chloroform) for the presence of product. The first 40 fractions contained starting material but no product and were discarded. Succeeding fractions which were found to contain the desired product were combined and evaporated to dryness. The residue was recrystallized from ethanol (with activated charcoal treatment) and from n-propanol to give 2-amino-9-(2-hydroxyethoxymethyl) adenine (0.5 g, 12% of theoretical), m.p. 183°-184° C.

Aufarbeitung

  1. customThe reaction mixture was removed from the bomb and excess ammonia
  2. customremoved in vacuo
  3. customThe residue was partitioned between ether and water
  4. washthe aqueous layer washed twice more with ether and once with chloroform
  5. additionThe aqueous solution was then treated with an excess of a strongly basic ion exchange resin
  6. customevaporated to dryness in vacuo
  7. dissolutionThe residue was dissolved in a minimal amount of ethanol, Florisil (Registered Trade Mark) (10g)
  8. additionadded
  9. customthe mixture evaporated in dryness
  10. washeluted with 5% methanol/95% chloroform (v/v)
  11. customFractions (30 ml) were collected
  12. customevaporated to dryness
  13. customThe residue was recrystallized from ethanol (with activated charcoal treatment) and from n-propanol

Quellen und Nachweise

ORD-Eintrag
ord-c0f4d0cdb588449989ec4f03538450ce
Quelldatensatz
uspto-grants-1981_10
Patentnummer
US04294831

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